Can killifish live with glofish?

Verdict
Yes

Yes. Killifish and glofish make a good pair. Both are fine at 72–77°F. Plan on at least 15 gal.

KillifishKillifish70–77°F · pH 5.5–7 GloFish tetraGloFish tetra72–80°F · pH 6–7.5

Why it works

Water side by side

55°60°65°70°75°80°85°90°Both: 72–77°Killifish 70–77°GloFish tetra 72–80°
The green band is the temperature range both can live in.
456789Both: 6–7Killifish 5.5–7GloFish tetra 6–7.5
The green band is the pH both tolerate.

Size to scale

Killifish · 2.5 inGloFish tetra · 2.5 in0"1"2"3"4"5"6"
Adult length in an aquarium. A fish can swallow anything that fits in its mouth, roughly a third to half its own length for predators.

Care at a glance

KillifishGloFish tetra
Temperature70–77 °F72–80 °F
pH5.5–76–7.5
Adult size2.5 in2.5 in
Minimum tank10 gal15 gal
TemperamentPeacefulSemi-aggressive
Swims atTopMiddle
GroupAlone or in pairsGroups of 6+
DietCarnivoreOmnivore

Better tank mates for killifish

All killifish tank mates

Better tank mates for glofish

All glofish tetra tank mates

Questions

Can killifish live with glofish?

Yes. Killifish and glofish make a good pair. Both are fine at 72–77°F. Plan on at least 15 gal.

What temperature suits both killifish and glofish?

72–77 °F. Killifish need 70–77 °F, glofish need 72–80 °F.

What size tank do killifish and glofish need together?

At least 15 gal, set by the glofish tetra. Add space for each extra fish.